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Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

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Improved double beam, vacuum far infrared spectrophotometer.

I Iwahashi, K Matsumoto, S Matsudaira

|January 15, 2010

Summary

This study enhanced a double beam far infrared spectrophotometer for improved performance and ease of use. The upgraded instrument offers versatile measurement options and high-resolution spectral analysis.

Area of Science:

Spectroscopy
Analytical Chemistry
Physical Chemistry

Background:

Far infrared (FIR) spectroscopy is crucial for molecular analysis.
Conventional spectrophotometers can be limited by water vapor interference and operational complexity.
Improvements in instrumentation are needed for enhanced performance and versatility.

Purpose of the Study:

To enhance a double beam far infrared spectrophotometer for improved convenience and performance.
To enable versatile measurements through multiple photometric systems.
To achieve high-resolution spectral analysis across a broad FIR range.

Main Methods:

The instrument was made evacuable, with an optional dry air purge for the sample chamber.
Three photometric systems were integrated: conventional double beam, double beam double chopping, and single beam.

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An efficient transmission filter system, avoiding deliquescent crystals, was implemented.

A refined control system enabled fully automatic operation.

Main Results:

The improved spectrophotometer offers enhanced convenience and higher performance.
Versatile measurements are possible using the selectable photometric systems.
High resolution is achieved across the entire spectral region from 400 cm(-1) to 30 cm(-1).
Fully automatic operation allows for a 30-minute scan over the entire spectral region.

Conclusions:

The enhanced double beam far infrared spectrophotometer provides significant operational advantages.
The instrument facilitates high-resolution spectral analysis with increased convenience.
This upgrade enables more efficient and versatile measurements in the far infrared region.