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Related Concept Videos

UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
UV–Vis Spectroscopy: Beer–Lambert Law01:09

UV–Vis Spectroscopy: Beer–Lambert Law

The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The modern...
Atomic Absorption Spectroscopy: Instrumentation01:22

Atomic Absorption Spectroscopy: Instrumentation

An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...
Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...
Atomic Absorption Spectroscopy: Interference01:25

Atomic Absorption Spectroscopy: Interference

Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...

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Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared
07:38

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Published on: January 10, 2025

Computer programs for absorption spectrophotometry.

R N Jones

    Applied Optics
    |January 15, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Twenty-two modular Fortran IV computer programs for absorption spectrophotometry data analysis are available. These programs offer standardized input/output for complex systems and cover diverse numerical computations for IR, UV, and visible spectrophotometry.

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    Published on: January 10, 2025

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    An Introduction to Processing, Fitting, and Interpreting Transient Absorption Data
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    An Introduction to Processing, Fitting, and Interpreting Transient Absorption Data

    Published on: February 16, 2024

    Area of Science:

    • Spectroscopy
    • Computational Chemistry
    • Analytical Chemistry

    Background:

    • Absorption spectrophotometry generates large datasets requiring sophisticated numerical analysis.
    • Existing methods for processing spectrophotometric data can be fragmented and difficult to integrate.
    • Standardized computational tools are essential for reproducible and efficient data analysis in spectrophotometry.

    Purpose of the Study:

    • To introduce a suite of twenty-two modular computer programs for numerical computations in absorption spectrophotometry.
    • To provide standardized input/output formats for easy interfacing and system integration.
    • To offer versatile tools applicable to infrared (IR), ultraviolet (UV), and visible spectrophotometry.

    Main Methods:

    • Development of modular programs written in Fortran IV.
    • Implementation of standardized input and output formats for data processing.
    • Inclusion of algorithms for unit conversions, smoothing, differentiation, band analysis, and spectral deconvolution.

    Main Results:

    • A comprehensive set of twenty-two modular programs covering essential numerical computations for absorption spectrophotometry.
    • Programs facilitate interconversions, data manipulation, band analysis (including overlapping bands), and slit function corrections.
    • Demonstrated applicability to IR, UV, and visible spectrophotometry with potential for further integration into complex systems.

    Conclusions:

    • The developed modular programs provide a powerful and flexible toolkit for absorption spectrophotometry data analysis.
    • Standardization of formats enhances interoperability and facilitates the creation of advanced data processing systems.
    • These Fortran IV programs offer a valuable resource for researchers in various spectroscopic fields.