Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Intensity Of Electromagnetic Waves01:22

Intensity Of Electromagnetic Waves

The energy transport per unit area per unit time, or the Poynting vector, gives the energy flux of an electromagnetic wave at any specific time. For a plane electromagnetic wave with E0 and B0 as the peak electric and magnetic fields and traveling along the x-axis, the time-varying energy flux can be given by the following equation:
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
Interference and Diffraction02:18

Interference and Diffraction

Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
X-ray Imaging01:24

X-ray Imaging

German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with X-rays, and by 1900, X-ray was widely...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Experimental fourier analysis of speckle patterns.

Applied optics·2010
Same author

Holographic subsonic flow visualization.

Applied optics·2010
Same author

Optimizing pulsed laser holographic nondestructive testing.

Applied optics·2010
Same author

Absolute measurements of absorption at the iodine-laser frequency in atmospheric gases.

Optics letters·2009
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jun 17, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

An Intensity MTF Theory for Coherent Imaging.

C J Reinheimer, C E Wiswall

    Applied Optics
    |January 15, 2010
    PubMed
    Summary
    This summary is machine-generated.

    An intensity modulation transfer function (MTF) can analyze coherent imaging systems with diffuse targets. This provides an alternative to amplitude modulation transfer functions for understanding image quality in these systems.

    More Related Videos

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
    20:00

    Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

    Published on: October 31, 2015

    Related Experiment Videos

    Last Updated: Jun 17, 2026

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
    12:54

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

    Published on: October 2, 2021

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
    20:00

    Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

    Published on: October 31, 2015

    Area of Science:

    • Optics and Photonics
    • Image Analysis
    • Coherent Imaging Systems

    Background:

    • Traditional analysis of holographic and coherent imaging systems relies on amplitude modulation transfer functions.
    • Understanding how system parameters affect image quality is crucial for optimizing performance.

    Purpose of the Study:

    • To demonstrate the utility of an intensity modulation transfer function (MTF) for analyzing coherent imaging systems.
    • To provide an alternative or complementary method to amplitude MTF for coherent imaging analysis.

    Main Methods:

    • Employing diffuse targets within the coherent imaging system.
    • Analyzing the system's response using an intensity modulation transfer function (MTF).
    • Evaluating the usable image area for analysis.

    Main Results:

    • The intensity modulation transfer function (MTF) effectively analyzes coherent systems under specific conditions.
    • This method is applicable when diffuse targets are used and the usable image area is considered.
    • The findings show a viable alternative for characterizing image quality.

    Conclusions:

    • An intensity modulation transfer function (MTF) offers a practical approach for analyzing coherent imaging systems.
    • The use of diffuse targets is key for the successful application of intensity MTF in these systems.
    • This research expands the toolkit for evaluating image quality in coherent optics.