Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Sferics and headache: a prospective study.

Cephalalgia : an international journal of headache·2001

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 17, 2026

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Published on: August 11, 2020

An asymmetry method for high precision alignment with laser light.

|January 15, 2010

Summary

A novel laser technique enables high-precision alignment of long rectilinear systems without complex optics. This method detects minute misalignments using diffraction-induced intensity asymmetries, offering a simple yet accurate solution for various applications.

Area of Science:

Optics and Photonics
Metrology
Precision Engineering

Background:

Precise alignment is critical for many scientific and industrial systems.
Existing laser alignment techniques often require high-resolution optics or have limited operational ranges.
A need exists for simpler, more flexible, and highly accurate alignment methods.

Purpose of the Study:

To introduce a new, simple, and highly accurate method for aligning rectilinear systems.
To demonstrate the capability of the method over extended operational ranges (0 to several kilometers).
To compare the advantages of this asymmetry method against conventional laser alignment techniques.

Main Methods:

Utilizing laser light with antisymmetrical transverse phase.

More Related Videos

The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry

The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry

Published on: August 12, 2013

Measurement of Tension Release During Laser Induced Axon Lesion to Evaluate Axonal Adhesion to the Substrate at Piconewton and Millisecond Resolution

Measurement of Tension Release During Laser Induced Axon Lesion to Evaluate Axonal Adhesion to the Substrate at Piconewton and Millisecond Resolution

Published on: May 27, 2013

Related Experiment Videos

Last Updated: Jun 17, 2026

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Published on: August 11, 2020

The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry

The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry

Published on: August 12, 2013

Measurement of Tension Release During Laser Induced Axon Lesion to Evaluate Axonal Adhesion to the Substrate at Piconewton and Millisecond Resolution

Measurement of Tension Release During Laser Induced Axon Lesion to Evaluate Axonal Adhesion to the Substrate at Piconewton and Millisecond Resolution

Published on: May 27, 2013

Generating intensity asymmetries through diffraction behind a misaligned target.

Employing a simple apparatus for both visual and automated detection in one or two directions.

Main Results:

Achieved extreme accuracy in alignment, detecting misalignments below 0.01 mm (10(-8) rad at 1 km).
Demonstrated operational range from zero to several kilometers.
Showcased the flexibility and simplicity of the developed apparatus.

Conclusions:

The described asymmetry method offers a significant advancement in precision alignment technology.
It provides a cost-effective and versatile alternative to existing laser alignment methods.
The technique has broad applicability in fields requiring precise system alignment.