Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The effect of raw soybean on the pancreas of adult dogs.

Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·1971
Same author

The effect of dietary soybean trypsin-inhibitor on the histology of dog pancreas.

Life sciences. Pt. 2: Biochemistry, general and molecular biology·1971
Same author

Effect of nicotinic acid on gastric secretion of acid in human subjects and in dogs.

Scandinavian journal of gastroenterology·1971
Same author

Effect of antrectomy on gastric secretion following duodenal exclusion.

The American journal of digestive diseases·1969
Same author

Relationship of bean substrates and certain intestinal bacteria to gas production in the dog.

Gastroenterology·1968
Same author

Production and inhibition of gas in various regions in the intestine of the dog.

Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·1966

Related Experiment Video

Updated: Jun 17, 2026

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

Limitations in optical imaging devices at low light levels.

E A Richards

    Applied Optics
    |January 15, 2010
    PubMed
    Summary

    Objective lens parameters significantly impact low light imaging resolution and perception range. Larger lenses reduce depth of field, posing trade-offs against quantum noise and optical limits for better low-light visibility.

    Area of Science:

    • Optical Engineering
    • Imaging Science
    • Photonics

    Background:

    • Low light level imaging is crucial for various applications, including surveillance and scientific research.
    • Understanding the factors limiting image quality in low light is essential for device design.

    Purpose of the Study:

    • To investigate how objective lens parameters affect the resolution of low light imaging devices.
    • To determine the relationship between perception range and incident light levels.
    • To analyze depth of field restrictions and compare them with noise and resolution limitations.

    Main Methods:

    • Analysis of objective lens characteristics.
    • Deduction of perception range based on incident light.
    • Investigation of depth of field limitations.

    More Related Videos

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
    10:07

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

    Published on: April 9, 2014

    Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
    10:16

    Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

    Published on: February 8, 2014

    Related Experiment Videos

    Last Updated: Jun 17, 2026

    Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
    20:00

    Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

    Published on: October 31, 2015

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
    10:07

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

    Published on: April 9, 2014

    Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
    10:16

    Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

    Published on: February 8, 2014

  • Comparison with quantum noise and optical resolution thresholds.
  • Discussion of image intensifier performance degradation due to dark noise.

    • Main Results:

    • Objective lens parameters directly influence the resolution capabilities of low light imaging systems.
    • Perception range is dependent on both lens parameters and incident light levels.
    • Larger lenses introduce depth of field limitations that must be balanced against noise and optical resolution.

    Conclusions:

    • Lens design is a critical factor in optimizing low light imaging performance.
    • Trade-offs exist between achieving high resolution, extended perception range, and sufficient depth of field.
    • Image tube dark noise significantly degrades the performance of image intensifiers.