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Related Experiment Video

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Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Published on: August 31, 2019

TAT-peptide modified liposomes: preparation, characterization, and cellular interaction.

Marjan M Fretz¹, Gert Storm

¹Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)

|January 15, 2010

Summary

This summary is machine-generated.

Liposomes carrying drugs often degrade before reaching their target. Modifying liposomes with TAT-peptides allows direct cell entry, bypassing degradation for improved drug delivery.

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Last Updated: Jun 17, 2026

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Published on: August 31, 2019

Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins

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Solid Lipid Nanoparticles (SLNs) for Intracellular Targeting Applications

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Published on: November 17, 2015

Area of Science:

Biotechnology and Nanomedicine
Cellular Biology and Drug Delivery

Background:

Macromolecular drugs encapsulated in liposomes typically enter cells via endocytosis.
The endosomal/lysosomal pathway can lead to the degradation of liposome-encapsulated contents.
Bypassing endocytosis is crucial for effective delivery of sensitive macromolecular drugs.

Purpose of the Study:

To develop liposomes with direct plasma membrane translocation capabilities.
To investigate the cellular interaction of TAT-peptide modified liposomes.
To enhance the delivery efficiency of liposome-encapsulated macromolecular drugs.

Main Methods:

Preparation of liposomes functionalized with TAT-peptides.
Utilizing live cell flow cytometry to analyze cellular uptake.
Employing live cell imaging techniques to visualize liposome-cell interactions.

Main Results:

Successful preparation of TAT-peptide modified liposomes.
Demonstrated cellular interaction and internalization of modified liposomes.
Evidence of potential for bypassing the endocytic pathway.

Conclusions:

TAT-peptide modification confers plasma membrane translocation to liposomes.
This strategy offers a promising approach to overcome endosomal degradation.
Enhanced liposomal drug delivery systems can be achieved through peptide functionalization.