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Related Concept Videos

Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
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Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...

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Related Experiment Video

Updated: Jun 17, 2026

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2
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Anionic pH sensitive lipoplexes.

Nathalie Mignet1, Daniel Scherman

  • 1Unité de Pharmacologie Chimique et Génétique, Inserm, U640, Paris, France.

Methods in Molecular Biology (Clifton, N.J.)
|January 15, 2010
PubMed
Summary

Researchers developed pH-sensitive anionic pegylated lipoplexes for gene delivery to tumors. These nanoparticles demonstrate optimized pH sensitivity for targeted release, enhancing gene therapy potential.

Area of Science:

  • Biotechnology
  • Nanomedicine
  • Gene Therapy

Background:

  • Developing effective gene delivery systems for cancer therapy remains a challenge.
  • Targeted delivery of genetic material to tumors requires overcoming biological barriers.
  • pH-sensitive nanoparticles offer potential for controlled drug release in tumor microenvironments.

Purpose of the Study:

  • To design and characterize novel anionic pegylated lipoplexes for enhanced gene delivery to tumors.
  • To achieve pH-sensitive properties for targeted release of genetic material within the tumor.
  • To optimize the formulation for efficient DNA complexation and in vitro transfection.

Main Methods:

  • Anionic pegylated lipoplexes were formulated by combining cationic lipoplexes and pegylated anionic liposomes.

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  • Light scattering was used to monitor particle surface charge neutralization and optimize lipid ratios for pH sensitivity (5.5-6.5).
  • DNA accessibility to picogreen was assessed to confirm DNA compaction within the complexes. In vitro transfection efficiency was evaluated using bafilomycin.

    • Main Results:

    • Optimized anionic pegylated lipoplexes exhibited pH sensitivity in the range of 5.5-6.5.
    • Successful compaction of DNA within the anionic complexes was confirmed.
    • In vitro studies demonstrated effective transfection and confirmed the pH-sensitive properties of the nanoparticles.

    Conclusions:

    • Anionic pegylated lipoplexes are a promising platform for pH-sensitive gene delivery to tumors.
    • The optimized formulation facilitates targeted gene delivery and release, potentially improving cancer therapy efficacy.
    • Further in vivo studies are warranted to validate the therapeutic potential of these nanoparticles.