Direct determination of tissue aminothiol, disulfide, and thioether levels using HPLC-ECD with a novel stable
Bruce Bailey1, John Waraska, Ian Acworth
1ESA Biosciences, Inc, Chelmsford, MA, USA. bbailey@esainc.com
Methods in Molecular Biology (Clifton, N.J.)
|January 15, 2010
Abstract:
This chapter describes two different methods using reversed-phase HPLC with electrochemical detection on a boron-doped diamond (BDD) working electrode for the direct, routine, sensitive and simultaneous measurement of a number of aminothiols, disulfides, and thioethers, in either plasma or tissue homogenates.
