Related Experiment Video
Updated: Jun 17, 2026

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
Separation of phenylpropanoids and evaluation of their antioxidant activity
Sammer Yousuf1, M Iqbal Choudhary, Atta Ur Rahman
1International Center for Chemical and Biological Sciences, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi, Pakistan.
Abstract:
Phenylpropanoids are a group of natural products with a wide range of biological and pharmacological importance. They have been isolated from a large number of plants by utilizing a diverse range of chromatographic techniques. We describe here the utilization of normal, reverse phase, and high pressure liquid column chromatographic techniques for the purification of phenylpropanoids from the crude plant extracts. Most important of them is the recycling reverse phase HPLC effectively utilized to purify the highly oxygenated phenylpropanoids glycosides from the butanolic and water extracts. The antioxidant activity of the different phenylpropanoids is also reported on the basis of DPPH, superoxide anion scavenging and Fe(2+)-chelating assays, along with electron spin resonance (ESR) method.
More Related Videos
14:39Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
Published on: April 22, 2022
07:05Microwave-Assisted Extraction of Phenolic Compounds and Antioxidants for Cosmetic Applications Using Polyol-Based Technology
Published on: August 23, 2024
Related Concept Videos
Oxidation of Phenols to Quinones
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox property is crucial in...
Phase I Reactions: Oxidation of Aliphatic and Aromatic Carbon-Containing Systems
Oxidation reactions are fundamental in aromatic carbon-containing systems. An example is the hydroxylation of phenobarbital, a process that transforms it into...
Acidity and Basicity of Alcohols and Phenols
Radical Autoxidation
Physical Properties of Alcohols and Phenols
Alcohols possess a higher boiling point than aliphatic hydrocarbons of similar...
Hydrolysis of Chlorobenzene to Phenol: Dow Process