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Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...

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Updated: Jun 17, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
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Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

[Recent advances in aptamer affinity chromatography].

Qiang Zhao1, Le X Chris

  • 1Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton T6G 2G3, Canada.

Se Pu = Chinese Journal of Chromatography
|January 16, 2010
PubMed
Summary

Aptamer affinity chromatography uses aptamers (single-stranded DNA or RNA molecules) for specific separation and concentration of targets. This technique offers advantages over antibodies for analyzing small molecules, proteins, and cells.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biotechnology

Context:

  • Aptamer affinity chromatography is an advanced separation technique utilizing aptamers as specific binding agents.
  • Aptamers, as single-stranded oligonucleotides, offer superior preparation, stability, and application versatility compared to traditional antibodies.
  • This method enables precise separation and concentration of diverse targets, including small molecules, proteins, and cells.

Purpose:

  • To review and summarize the current applications of aptamer affinity chromatography in separation science.
  • To critically evaluate the existing literature on aptamer-based chromatographic methods.
  • To discuss the future potential and prospective developments in aptamer affinity chromatography.

Summary:

  • Aptamer affinity chromatography employs aptamers, which are nucleic acid-based ligands, for highly specific molecular recognition and purification.
  • The review covers the successful implementation of this technique for the analysis and isolation of small molecules, proteins, and cellular targets.
  • Key advantages of aptamers, such as ease of synthesis and enhanced stability, are highlighted in the context of chromatographic applications.

Impact:

  • Provides a comprehensive overview of the state-of-the-art in aptamer affinity chromatography.
  • Identifies current trends and challenges, paving the way for future research and development.
  • Highlights the potential of aptamer affinity chromatography to advance analytical methodologies in various scientific fields.