Related Experiment Video
Updated: Jun 17, 2026
11:00
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
Published on: May 20, 2013
[Progress of on-line sample preparation techniques in chromatographic analysis]
Qisheng Zhong1, Yufei Hu, Gongke Li
1School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
Se Pu = Chinese Journal of Chromatography
|January 16, 2010
Summary
On-line sample preparation techniques are crucial for chromatographic analysis, reducing time and errors. This review covers advancements in methods like solid phase extraction and microfluidic chips for improved analytical chemistry.
Area of Science:
- Analytical Chemistry
- Chromatography
Context:
- Sample preparation is a critical, time-consuming, and error-prone step in chromatographic analysis.
- Developing efficient on-line sample preparation techniques is a major focus in analytical chemistry.
Purpose:
- To review the progress and various techniques of on-line sample preparation in chromatographic analysis.
- To highlight advancements in automated sample handling for chromatography.
Summary:
- This paper reviews key on-line sample preparation techniques including solid phase extraction (SPE), solid phase microextraction (SPME), liquid phase microextraction (LPME), membrane assisted extraction, field assisted extraction, gas phase extraction, thermal desorption, and microfluidic chip technologies.
- These methods aim to streamline the sample preparation process for chromatographic analysis.
Impact:
- Improved efficiency and accuracy in chromatographic analysis.
- Reduced sample preparation time and potential for human error.
- Facilitates the adoption of automated and high-throughput analytical workflows.
Related Concept Videos
01:21Sample Preparation for Analysis: Overview
Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
01:08Sample Preparation for Analysis: Advanced Techniques
Accurate analysis of complex samples often requires advanced preparation techniques to achieve reliable and reproducible results. Samples containing inorganic or organic materials can be challenging to dissolve or decompose effectively. Standard sample preparation methods include acid digestion, fusion, dry ashing, and wet digestion.
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...
01:13Principles Of Column Chromatography
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
01:10Chromatography: Introduction
Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
01:12Chromatographic Methods: Classification
Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
Chromatographic techniques are typically named by...
01:05High-Performance Liquid Chromatography: Elution Process
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...