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Regulated mRNA Transport

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...

Regulated mRNA Transport

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...

Nuclear Export of mRNA

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...

Nuclear Export of mRNA

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...

Nuclear Export

Nuclear Export

The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...

Nucleic Acid Structure

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...

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Related Experiment Video

Updated: Jun 17, 2026

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

Structure and function of mRNA export adaptors.

Matthew J Walsh¹, Guillaume M Hautbergue, Stuart A Wilson

¹Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK.

Biochemical Society Transactions

|January 16, 2010

Summary

This summary is machine-generated.

mRNA export adaptors link nuclear processing to export receptors like TAP/NXF1. These adaptors integrate mRNA information, license it for export, and ensure stable binding to the export receptor.

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Area of Science:

Molecular Biology
Cell Biology
Biochemistry

Background:

mRNA export adaptors are crucial for linking nuclear mRNA processing to the export machinery.
They integrate signals from transcriptional and post-transcriptional events to regulate mRNA export.
The export receptor complex, including TAP/NXF1/Mex67p, mediates the transport of mRNA out of the nucleus.

Purpose of the Study:

To discuss the structure and function of mRNA export adaptors.
To elucidate the mechanisms by which adaptors are recruited to mRNA.
To explain how adaptors facilitate the handover of mRNA to the TAP/NXF1/Mex67p export receptor.

Main Methods:

The study is a discussion/review of existing literature.
It synthesizes information on adaptor protein structure and function.
It integrates data on the recruitment of adaptors to mRNA and their interaction with export factors.

Main Results:

mRNA export adaptors act as crucial intermediaries, integrating mRNA processing information.
They are recruited to mRNA via both transcriptional and post-transcriptional mechanisms.
Adaptors facilitate a higher-affinity RNA-binding state for the TAP/NXF1/Mex67p complex, stabilizing mRNA export.

Conclusions:

mRNA export adaptors are essential for efficient and regulated mRNA export from the nucleus.
Their recruitment and function are tightly controlled, ensuring fidelity in the export process.
Understanding adaptor structure and function provides insights into gene expression regulation and nuclear transport.