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Related Concept Videos

Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Coupling genetics and post-genomic approaches to decipher the cellular splicing code at a systems-wide level.

Yilei Liu1, David J Elliott

  • 1Institute of Human Genetics, Newcastle University, Newcastle upon Tyne NE1 3BZ, UK.

Biochemical Society Transactions
|January 16, 2010
PubMed
Summary

Nuclear RNA processing, essential for gene expression, is regulated by RNA-binding proteins. These proteins control alternative splicing, creating diverse messenger RNAs (mRNAs) and revealing complex gene expression networks.

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Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Related Experiment Videos

Last Updated: Jun 17, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Nuclear RNA processing is a crucial step in eukaryotic gene expression.
  • The concentration and expression of nuclear RNA-binding proteins significantly influence this process.
  • Differential expression of these proteins in various cell types contributes to alternative splicing of pre-mRNAs into distinct mRNAs.

Purpose of the Study:

  • To explore the complexity of nuclear RNA processing using recent post-genomic technologies.
  • To elucidate the mechanisms and regulatory rules governing networks of RNA-binding proteins.
  • To understand how these networks regulate multiple parallel RNA processing pathways.

Main Methods:

  • Utilized advanced post-genomic technologies to analyze nuclear RNA processing.
  • Investigated the role of differentially expressed nuclear RNA-binding proteins.
  • Examined the regulation of alternative splicing pathways.

Main Results:

  • Revealed the intricate complexity of nuclear RNA processing.
  • Identified mechanisms by which RNA-binding protein networks regulate parallel processing pathways.
  • Demonstrated differential expression of RNA-binding proteins across cell types.

Conclusions:

  • Nuclear RNA-binding proteins are key regulators of alternative splicing and gene expression.
  • Networks of RNA-binding proteins orchestrate multiple parallel RNA processing pathways.
  • Systems-wide understanding of alternative nuclear RNA processing is emerging.