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Related Experiment Video

Updated: Jun 17, 2026

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

Published on: November 11, 2025

Nuclear envelope influences on genome organization.

Poonam Malik¹, Nikolaj Zuleger, Eric C Schirmer

¹Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK.

Biochemical Society Transactions

|January 16, 2010

Summary

This summary is machine-generated.

The nuclear periphery influences genome organization and gene regulation. Research shows genes can be artificially tethered to the nuclear periphery, with future work focusing on identifying involved proteins.

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Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

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3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

Published on: January 25, 2020

Area of Science:

Cell Biology
Molecular Biology
Genetics

Background:

The nuclear periphery is crucial for genome organization and gene regulation.
Specific chromosome and gene positioning within the nucleus is heritable and tissue-specific.
Genes at the nuclear periphery are often inactive, but can be reactivated upon release.

Purpose of the Study:

To investigate the role of the nuclear periphery in genome organization and gene regulation.
To explore the potential for artificial tethering of genes to the nuclear periphery.
To identify endogenous nuclear envelope and chromatin proteins involved in affinity-driven NE tethering.

Main Methods:

Utilizing specially designed experimental systems.
Employing affinity-based mechanisms for gene tethering.
Investigating gene activity changes in relation to nuclear positioning.

Main Results:

Demonstrated that genes can be artificially tethered to the nuclear periphery using an affinity mechanism.
Observed that peripheral gene localization is associated with gene inactivity.
Showed that releasing genes from the periphery can reverse their inactive state.

Conclusions:

The nuclear periphery plays a significant role in regulating gene expression.
Artificial tethering provides a tool to study NE-chromatin interactions.
Identifying the specific proteins involved is the next critical step for understanding NE tethering regulation.