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Phage typing of Staphylococcus intermedius.

G D Overturf1, D A Talan, K Singer

  • 1Department of Pathology, University of California, Los Angeles.

Journal of Clinical Microbiology
|February 1, 1991
PubMed
Summary
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Lysogenic phages are common in Staphylococcus intermedius, a canine pathogen. Phage typing showed varied lytic patterns, suggesting its utility in differentiating related bacterial strains.

Area of Science:

  • Veterinary Microbiology
  • Bacteriology
  • Molecular Biology

Background:

  • Staphylococcus intermedius is a significant canine pathogen and an infrequent cause of human infections.
  • Understanding the genetic diversity and epidemiological links of S. intermedius strains is crucial for both veterinary and public health.

Purpose of the Study:

  • To investigate the prevalence of lysogenic phages in Staphylococcus intermedius strains.
  • To evaluate the potential of phage typing as a tool for differentiating S. intermedius isolates from various sources.

Main Methods:

  • Screening of 145 S. intermedius isolates (including canine infections, gingival samples, human isolates, and reference strains) for lysogenic phages using a modified Fisk method.
  • Preparation of 19 phage lysates for preliminary typing experiments.

Related Experiment Videos

  • Analysis of lytic activity and determination of lytic patterns for each isolate.
  • Main Results:

    • Lytic activity was detected in 64.1% (93/145) of S. intermedius isolates, revealing 44 distinct lytic patterns.
    • Lytic patterns did not differentiate between canine and human wound isolates versus canine gingival isolates.
    • Human wound isolates matched common canine patterns, while a nasopharyngeal isolate was non-lysed.

    Conclusions:

    • Lysogenic phages are prevalent in Staphylococcus intermedius.
    • Phage typing demonstrates potential as a valuable method for distinguishing epidemiologically related strains of S. intermedius.