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Updated: Jun 17, 2026
13:21
Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
Published on: May 4, 2012
Glycoprotein analysis using protein microarrays and mass spectrometry
Tasneem Patwa1, Chen Li, Diane M Simeone
1Pfizer Global Research, Groton, CT, USA.
Mass Spectrometry Reviews
|January 16, 2010
Summary
Protein glycosylation is vital for biological processes and disease diagnosis. Microarray platforms offer advanced tools for studying complex glycans and glycoproteins, aiding biomarker discovery.
Area of Science:
- Biochemistry
- Glycobiology
- Molecular Biology
Background:
- Protein glycosylation is crucial for cellular functions and disease progression, presenting significant research challenges due to carbohydrate complexity.
- Changes in glycosylation patterns are linked to diseases like cancer and inflammation, highlighting their diagnostic potential.
Purpose of the Study:
- To review experimental methodologies for studying glycosylation.
- To emphasize the role and advances of microarray platforms in glycoprotein and glycan analysis.
- To discuss the application of microarrays in cell characterization and biomarker discovery.
Main Methods:
- Survey of experimental techniques for glycosylation studies.
- Focus on microarray platforms, including glycoprotein, glycan, lectin, and antibody/lectin arrays.
- Utilizing probes for interrogating glycosylated molecules on arrays for structural characterization and biological interaction studies.
Main Results:
- Advancements in isolation, separation, and characterization of carbohydrates and glycoproteins.
- Microarray platforms provide high-throughput and reproducible analysis for biomarker validation.
- Recent progress in various microarray formats for glycoprotein analysis.
Conclusions:
- Microarray platforms are essential tools for characterizing glycan structure and studying glycosylation-related interactions.
- These platforms facilitate biomarker discovery and cell characterization.
- Continued development of microarray technologies enhances the study of glycosylation.
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