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Updated: Jun 17, 2026

Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes
Published on: August 31, 2022
[Genetic characteristic of wild-type measles viruses isolated in Ningbo from 2004 to 2008]
Yan Fu1, Hong-Xia Ni, Jian-Yi Liu
1Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, Zhejiang, China.
Objective:
To investigate the genetic characterizations and genotypes of measles viruses that was prevalent in Ningbo city.
Methods:
Measles viruses were isolated from throat swab specimens of suspected measles cases from 2004 to 2008 and 456 bp fragments of C terminus of nucleprotein (N) gene were amplified by RT-PCR. Sequence analysis was conducted of all 22 virus strains while compared with other measles virus strain which published in GenBank.
Results:
All of the 22 measles isolates belonged to genotype H1. The homogeneities of 456 bp fragments of C terminus of nucleprotein (N) gene were as high as 97.1%-98.5%,98.2%-99.6% and 98.5%-98.9% respectively. The nucleotide (amino acids) variability was 8.0%-9.5% (9.8%-14.5%) compared to S191.
Conclusion:
Genotype H1 measles virus circulated in Ningbo, China from 2004 to 2008. H1a was the predominant epidemic strain, H1b strain was existed as well.
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