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Updated: Jun 17, 2026

Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage (CoTrAC)
11:31

Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage (CoTrAC)

Published on: March 15, 2013

The transforming acidic coiled coil (TACC1) protein modulates the transcriptional activity of the nuclear receptors

Romain Guyot1, Séverine Vincent, Julie Bertin

  • 1Institut de Génomique Fonctionnelle de Lyon, Universitéde Lyon, Université Lyon 1, CNRS, INRA, Ecole Normale Supérieure de Lyon, 46 allée d'Italie, 69364 Lyon Cedex 07, France.

BMC Molecular Biology
|January 19, 2010
PubMed
Abstract

Insights

TACC1, a novel nuclear hormone receptor (NR) coregulator, acts as a scaffold protein. Its interaction with NRs influences transcriptional activity and may play a role in tumor development.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Oncology

Background:

  • Nuclear hormone receptors (NRs) regulate transcription via coactivator/corepressor interactions.
  • Misregulated expression or localization of cofactors is implicated in cancer.
  • These cofactors may be involved in oncogenesis.

Purpose of the Study:

  • To identify and characterize novel NR coregulators.
  • To investigate the role of TACC1 in NR-mediated transcription.
  • To explore the potential involvement of TACC1 in cancer.

Main Methods:

  • Yeast two-hybrid screening
  • GST pulldown assays
  • Colocalization studies
  • Co-immunoprecipitation
  • TACC1 depletion experiments

Main Results:

  • Identified TACC1 as a novel NR coregulator interacting with TR and other NRs.
  • TACC1 preferentially binds to unliganded NRs and localizes to the nucleus.
  • TACC1 depletion reduced ligand-dependent transcriptional activity and altered TR localization.

Conclusions:

  • TACC1 functions as a scaffold protein for NR transcriptional complexes.
  • This scaffolding role suggests TACC1's involvement in tumor development.

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