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Interaction of heparin with cationic molecular probes: probe charge is a major determinant of binding stoichiometry
Helga Szelke1, Sarah Schübel, Job Harenberg
1University of Heidelberg, Inorganic Chemistry Institute, 69120 Heidelberg, Germany.
Abstract:
Fluorescent perylenediimide probes modified with 2, 4, 6, or 8 ammonium groups were synthesized and their binding to the antithrombotic drug heparin was studied by fluorescence spectroscopy in solution. The polyanionic polysaccharide strands of heparin bind more probe molecules per sugar unit when the charge of the latter is low, and stability of the probe-heparin complex increases with increasing probe charge.
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