Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Diversity of Protists IV

Diversity of Protists IV

Amoebozoa represent a diverse group of terrestrial and aquatic protists that utilize lobe-shaped pseudopodia for locomotion and feeding. This characteristic differentiates them from the Rhizaria, which possess threadlike pseudopodia. The primary classifications within Amoebozoa include gymnamoebas, entamoebas, and the plasmodial and cellular slime molds. Phylogenetic evidence indicates that Amoebozoa diverged from a lineage that ultimately gave rise to fungi and animals.Gymnamoebas and...

Diversity of Protists II

Diversity of Protists II

Alveolates are a group of organisms recognized by the presence of alveoli, which are cytoplasmic sacs located beneath the cell membrane. While their function remains uncertain, alveoli may help regulate water balance by controlling how much water enters and leaves the cell. In dinoflagellates, these structures may serve as armor plates. There are three major types of alveolates: ciliates, which move using cilia; dinoflagellates, which use flagella for movement; and apicomplexans, which are...

Diversity of Protists I

Diversity of Protists I

Excavata is a diverse group of protists that includes both chemoorganotrophic and phototrophic species, with some thriving in anaerobic environments. Among the key groups within Excavata are diplomonads and parabasalids, which are flagellated protists that lack mitochondria and chloroplasts. These microorganisms typically inhabit anoxic environments, such as the intestines of animals, where they exist either symbiotically or as parasites, relying on fermentation for energy production. Some...

Archaeal Cell Wall

Archaeal Cell Wall

Archaeal cell walls are structurally and compositionally distinct from their bacterial counterparts, lacking the characteristic peptidoglycan layer found in most bacteria. Instead, archaeal cell walls exhibit remarkable diversity, utilizing materials such as pseudomurein, polysaccharides, and proteins to construct their protective outer layers. This structural flexibility is closely tied to archaea's ecological adaptability.S-Layers: The Common Archaeal Cell WallThe S-layer is the most...

Prokaryotic Cells

Prokaryotic Cells

Prokaryotes are small unicellular organisms that include the domains—Archaea and Bacteria. Bacteria include many common organisms, such as Salmonella and E. coli, while the Archaea include extremophiles that live in harsh environments, such as volcanic springs.Like eukaryotic cells, all prokaryotic cells are surrounded by a plasma membrane, have genetic material in the form of single, circular DNA, a cytoplasm that fills the interior of the cell, and ribosomes that synthesize proteins. However,...

Prokaryotic Cells

Prokaryotic Cells

Prokaryotes are small unicellular organisms that include the domains — Archaea and Bacteria. Bacteria include many common microorganisms, such as Salmonella and E. coli, while the Archaea include extremophiles that live in harsh environments, such as volcanic springs.
Like eukaryotic cells, all prokaryotic cells are surrounded by a plasma membrane, have genetic material in the form of single, circular DNA, a cytoplasm that fills the interior of the cell, and ribosomes that synthesize proteins.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Sovereign AI supercomputers: a global landscape review of unprecedented biomedical research infrastructure.

Frontiers in artificial intelligence·2026

Same author

A genome-wide genetic screen identifies a novel kDNA replication protein in trypanosomes.

Nucleic acids research·2026

Same author

Re-maturation of canine chemically-induced hepatic progenitor cells toward biliary epithelial cells.

The Journal of veterinary medical science·2026

Same author

Control of retrotransposon-driven activation of the interferon response by the double-stranded RNA binding protein DGCR8.

Nucleic acids research·2026

Same author

Stunning Intricacies of RNA Editing Complexes RECC, RESC, and REH2C: Functional Organization, Developmental Regulation, and Evolutionary History in Kinetoplastid Protists.

Wiley interdisciplinary reviews. RNA·2026

Same author

Genome editing across Dictyostelia species enables comparative functional genetics of social amoebas.

Scientific reports·2026

Search research articles

Related Experiment Video

Updated: Jun 17, 2026

High-throughput Measurement of Dictyostelium discoideum Macropinocytosis by Flow Cytometry

High-throughput Measurement of Dictyostelium discoideum Macropinocytosis by Flow Cytometry

Published on: September 10, 2018

A new Dictyostelium prestalk cell sub-type.

Yoko Yamada¹, Robert R Kay, Gareth Bloomfield

¹School of Life Sciences, University of Dundee, Dow St., Dundee DD3 5EH, UK.

Developmental Biology

|January 19, 2010

Summary

Researchers identified a new Dictyostelium prestalk cell subtype, pstU, involved in fruiting body formation. They discovered that MybE and DimB transcription factors negatively regulate some genes while positively regulating others, revealing alternative differentiation pathways.

More Related Videos

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation

Published on: November 9, 2017

Studying the Protein Quality Control System of D. discoideum Using Temperature-controlled Live Cell Imaging

Studying the Protein Quality Control System of D. discoideum Using Temperature-controlled Live Cell Imaging

Published on: December 2, 2016

Related Experiment Videos

Last Updated: Jun 17, 2026

High-throughput Measurement of Dictyostelium discoideum Macropinocytosis by Flow Cytometry

High-throughput Measurement of Dictyostelium discoideum Macropinocytosis by Flow Cytometry

Published on: September 10, 2018

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation

Published on: November 9, 2017

Studying the Protein Quality Control System of D. discoideum Using Temperature-controlled Live Cell Imaging

Studying the Protein Quality Control System of D. discoideum Using Temperature-controlled Live Cell Imaging

Published on: December 2, 2016

Area of Science:

Cellular and Molecular Biology
Developmental Biology
Dictyostelium discoideum research

Background:

Dictyostelium development involves specialized prestalk cells for fruiting body construction.
The prototypic prestalk gene ecmA requires DimB and MybE transcription factors for DIF-1 inducibility.
The precise nature and function of prestalk cell subtypes, including anterior-like cells (ALCs), remain unclear.

Purpose of the Study:

To identify genes regulated by MybE and DimB in response to DIF-1.
To characterize novel prestalk cell subtypes and their differentiation pathways.
To investigate the role of MybE and DimB in regulating gene expression during Dictyostelium development.

Main Methods:

Genome-wide microarray analyses were performed on parental, mybE-, and dimB- Dictyostelium cells.
Reporter gene fusions (rtaA:gal(u), ecmA, ecmB) were used to track cell populations.
Analysis of gene expression and cell differentiation in wild-type and mutant strains.

Main Results:

MybE and DimB regulate a large set of DIF-1 inducible genes, with some under positive and others under negative control.
A novel prestalk cell subtype, pstU, was identified, characterized by rtaA expression and preferential localization to the upper cup.
PstU cell differentiation occurs normally in DIF-deficient mutants, indicating an alternative DIF-1 activation pathway.

Conclusions:

MybE and DimB exhibit dual roles in regulating DIF-1 inducible genes during Dictyostelium development.
The pstU cell subtype represents a newly defined prestalk cell population with specific developmental functions.
An alternative DIF-1 activation pathway exists for pstU differentiation, independent of MybE and DimB under certain conditions.