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Expression and prognostic values of Id-1 and Id-3 in gastric adenocarcinoma
Hai-Yan Yang1, Hao-Ling Liu, Gai-Yun Liu
1Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, P. R. Chin.
Background:
Id (inhibitor of differentiation/DNA binding)-1 and -3 are involved in neoangiogenesis; they antagonize basic helix-loop-helix proteins, inhibit differentiation, and enhance cell proliferation. The aim of this study was to investigate Id-1 and -3 expression in gastric tumors and their clinical relevance in gastric cancer.
Materials And Methods:
We investigated Id-1 and Id-3 expression in gastric cancer samples by immunohistochemistry and Western blotting, and further analyzed the relationship between expression of Id-1 and Id-3 and clinicopathologic characteristics.
Results:
Expression of Id-1 and -3 was found significantly more often in gastric cancers than in matched adjacent nonmalignant tissues. Cancer samples with poor or moderate histologic differentiation showed significantly stronger Id-1 and -3 expression than cancer samples with high differentiation. In cancer samples, strong or moderate expression of Id-3, but not Id-1, was a strong independent predictor for shorter overall survival in multivariate analysis.
Conclusions:
The level of Id-1 and -3 protein expression was associated with the malignant potential of gastric tumors. In cancer samples, stronger Id-1 and -3 expression is associated with poor differentiation and more aggressive behavior of tumor cells, resulting in poor clinical outcome. Consequently, Id-3 might be used to independently predict survival of patients with gastric cancer.
Insights
Inhibitor of differentiation (Id)-1 and -3 proteins are elevated in gastric cancers, correlating with poor differentiation and aggressive tumor behavior. Id-3 expression independently predicts shorter survival in gastric cancer patients.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Id (inhibitor of differentiation/DNA binding)-1 and -3 proteins are implicated in neoangiogenesis, inhibiting differentiation and promoting cell proliferation.
- Their role in gastric tumorigenesis requires further investigation.
Purpose of the Study:
- To investigate the expression of Id-1 and Id-3 in gastric tumors.
- To determine the clinical relevance and prognostic significance of Id-1 and Id-3 in gastric cancer.
Main Methods:
- Immunohistochemistry and Western blotting were used to analyze Id-1 and Id-3 expression in gastric cancer samples.
- Correlation analysis was performed between Id protein expression and clinicopathologic characteristics.
Main Results:
- Id-1 and Id-3 expression was significantly higher in gastric cancers compared to adjacent nonmalignant tissues.
- Stronger Id-1 and Id-3 expression was observed in poorly and moderately differentiated gastric cancers.
- Strong Id-3 expression, but not Id-1, independently predicted shorter overall survival in multivariate analysis.
Conclusions:
- Id-1 and Id-3 protein levels are associated with the malignant potential of gastric tumors.
- Elevated Id-1 and Id-3 expression correlates with poor differentiation and aggressive tumor behavior, leading to poorer clinical outcomes.
- Id-3 may serve as an independent prognostic biomarker for predicting survival in gastric cancer patients.