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Updated: Jun 17, 2026
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Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels
Published on: December 13, 2024
Eicosanoid-induced store-operated calcium entry in dendritic cells
Kiyoshi Itagaki1, Beverly E Barton, Thomas F Murphy
1Division of Trauma, Department of Surgery, University of Medicine and Dentistry of New Jersey-New Jersey Medical School, Newark, New Jersey 07101, USA.
The Journal of Surgical Research
|January 19, 2010
Summary
Eicosanoids, such as leukotriene B4 and LTD4, influence dendritic cell (DC) calcium levels by depleting endoplasmic reticulum stores. This finding reveals a novel mechanism for regulating DC function and immune responses.
Area of Science:
- Immunology
- Cell Biology
- Calcium Signaling
Background:
- Eicosanoids modulate immune cells, including dendritic cells (DCs).
- Dendritic cell maturation and function are influenced by store-operated calcium entry (SOCE).
- Intracellular calcium dynamics may mediate eicosanoid effects on DCs.
Purpose of the Study:
- To investigate the impact of eicosanoids on calcium mobilization in dendritic cells.
- To determine if eicosanoids affect store-operated calcium entry (SOCE) in DCs.
- To explore the role of intracellular calcium in eicosanoid-mediated DC function.
Main Methods:
- Dendritic cells (DCs) were generated from mouse bone marrow.
- The effects of eicosanoids on intracellular calcium levels and SOCE were measured.
- RT-PCR was used to identify relevant receptors and ion channels in DCs.
Main Results:
- Leukotriene B4 (LTB4) and LTD4 depleted calcium from DC endoplasmic reticulum stores.
- Specific antagonists blocked LTB4 and LTD4-induced calcium store depletion.
- DCs express receptors for LTB4 and cysteinyl-leukotrienes, along with TRPC and STIM1 proteins involved in SOCE.
- Prostaglandin E2 (PGE2) did not affect DC calcium mobilization.
Conclusions:
- Eicosanoids exhibit distinct effects on dendritic cell calcium mobilization.
- These findings suggest a novel mechanism for regulating DC maturation via calcium signaling.
- This has significant implications for understanding immune and non-immune inflammatory processes.
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