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Related Concept Videos

The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...

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Related Experiment Video

Updated: Jun 17, 2026

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
11:02

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Published on: May 27, 2016

EBNA1 regulates cellular gene expression by binding cellular promoters.

Allon Canaan1, Izhak Haviv, Alexander E Urban

  • 1Department of Genetics, Yale Cancer Center, Yale University School of Medicine, New Haven, CT 06520, USA. allon.canaan@yale.edu

Proceedings of the National Academy of Sciences of the United States of America
|January 19, 2010
PubMed
Summary

Epstein-Barr virus nuclear antigen 1 (EBNA1) binds cellular DNA, altering host gene expression in B and epithelial cells. This binding reveals a novel DNA motif, distinct from viral binding sites, impacting EBV-associated cancers.

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Related Experiment Videos

Last Updated: Jun 17, 2026

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
11:02

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Published on: May 27, 2016

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
10:16

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

Area of Science:

  • Virology
  • Molecular Biology
  • Oncology

Background:

  • Epstein-Barr virus (EBV) is linked to various cancers, including lymphomas and nasopharyngeal carcinoma.
  • EBV nuclear antigen 1 (EBNA1) is crucial for EBV latency and transformation, mediating viral DNA replication and gene regulation.
  • EBNA1's function relies on its DNA-binding capability.

Purpose of the Study:

  • To investigate the impact of EBNA1 on host cellular gene expression.
  • To identify cellular genes directly regulated by EBNA1.
  • To characterize the DNA-binding properties of EBNA1 on cellular promoters.

Main Methods:

  • Microarray analysis was performed on BJAB (B cell) and 293 (epithelial) cell lines transfected with EBNA1.
  • Chromatin immunoprecipitation (ChIP) was used to identify EBNA1 binding sites on cellular DNA.
  • Sequence analysis was conducted on enriched EBNA1-bound promoters.

Main Results:

  • EBNA1 expression induced distinct changes in cellular gene expression profiles in both BJAB and 293 cells.
  • ChIP assays confirmed direct EBNA1 binding to cellular promoters.
  • Sequence analysis identified a novel DNA motif recognized by EBNA1 on cellular promoters, differing from its viral binding site.

Conclusions:

  • EBNA1 directly influences host cellular gene expression by binding to specific DNA promoter regions.
  • The identification of a novel EBNA1 cellular DNA-binding motif provides new insights into EBV's oncogenic mechanisms.
  • Understanding EBNA1's interaction with host DNA is critical for developing targeted therapies against EBV-associated malignancies.