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Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...

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Related Experiment Video

Updated: Jun 17, 2026

Deferred Growth Inhibition Assay to Quantify the Effect of Bacteria-derived Antimicrobials on Competition

Deferred Growth Inhibition Assay to Quantify the Effect of Bacteria-derived Antimicrobials on Competition

Published on: September 3, 2016

Rapid and specific method for evaluating Streptomyces competitive dynamics in complex soil communities.

Daniel C Schlatter¹, Deborah A Samac, Mesfin Tesfaye

¹Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, Minnesota 551081, USA.

Applied and Environmental Microbiology

|January 19, 2010

Summary

New quantitative PCR (qPCR) assays precisely measure pathogenic Streptomyces scabiei and antibiotic-producing Streptomyces lavendulae in soil. These tools aid in understanding streptomycete competition within complex microbial communities.

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Last Updated: Jun 17, 2026

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Area of Science:

Microbiology
Soil Science
Molecular Biology

Background:

Studying microbial interactions in soil is challenging due to difficulties in quantifying specific populations.
Understanding the roles of individual species, like Streptomyces, is crucial for soil health and function.

Purpose of the Study:

To develop quantitative PCR (qPCR) assays for specific soil Streptomyces species.
To enable accurate quantification of pathogenic Streptomyces scabiei and antibiotic-producing Streptomyces lavendulae in complex soil microbial communities.

Main Methods:

Quantitative PCR (qPCR) assay development.
Application of qPCR for detecting and quantifying target Streptomyces species in soil samples.

Main Results:

Successfully developed and validated qPCR assays for Streptomyces scabiei and Streptomyces lavendulae.
Demonstrated the utility of these assays in quantifying target populations within diverse soil microbial communities.

Conclusions:

The developed qPCR assays provide a reliable method for quantifying specific Streptomyces populations in soil.
These assays are valuable tools for investigating the ecological dynamics and competitive interactions of streptomycetes in soil environments.