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Detection of 2,4,6-trinitrotoluene-utilizing anaerobic bacteria by 15N and 13C incorporation
Erin M Gallagher1, Lily Y Young, Lora M McGuinness
1Department of Environmental Sciences, the State University of New Jersey, New Brunswick, New Jersey 08901, USA.
Applied and Environmental Microbiology
|January 19, 2010
Abstract:
2,4,6-Trinitrotoluene ((15)N or (13)C labeled) was added to Norfolk Harbor sediments to test whether anaerobic bacteria use TNT for growth. Stable-isotope probing (SIP)-terminal restriction fragment length polymorphism (TRFLP) detected peaks in the [(15)N]TNT cultures (60, 163, and 168 bp). The 60-bp peak was also present in the [(13)C]TNT cultures and was related to Lysobacter taiwanensis.
