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Updated: Jun 17, 2026

Adaptation at the Extremes of Life: Experimental Evolution with the Extremophile Archaeon Sulfolobus acidocaldarius
Published on: June 14, 2024
Halostagnicola kamekurae sp. nov., an extremely halophilic archaeon from solar salt
Shuhei Nagaoka1, Hiroaki Minegishi2, Akinobu Echigo2
1Department of Biological Applied Chemistry, Graduate School of Engineering, Toyo University, 2100 Kujirai, Kawagoe, Saitama 350-8585, Japan.
Abstract:
A novel extremely halophilic archaeon, strain 194-10(T), was isolated from a solar salt sample imported into Japan from the Philippines. Strain 194-10(T) was pleomorphic, neutrophilic and mesophilic and required at least 10 % (w/v) NaCl but no MgSO(4) . 7H(2)O for growth; it exhibited optimal growth at 15 % (w/v) NaCl and 60 mM MgSO(4) . 7H(2)O. Strain 194-10(T) grew at 20-45°C (optimum, 30°C) and pH 6.0-9.0 (optimum, pH 6.5-7.0). The G+C content of its DNA was 59.8 mol%. 16S rRNA gene sequence analysis revealed closest proximity to Halostagnicola larsenii XH-48(T) (98.5 % similarity), the sole representative of the genus Halostagnicola. Polar lipid analysis revealed that strain 194-10(T) contained phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester (the latter derived from both C(20)C(20) and C(20)C(25) archaeol) and several unidentified glycolipids. The results of DNA-DNA hybridization (20.7 % relatedness between Hst. larsenii JCM 13463(T) and strain 194-10(T)) and physiological and biochemical characteristics allowed differentiation of strain 194-10(T) from Hst. larsenii XH-48(T). Therefore, strain 194-10(T) represents a novel species of the genus Halostagnicola, for which the name Halostagnicola kamekurae sp. nov. is proposed, with the type strain 194-10(T) (=DSM 22427(T) =JCM 16110(T) =CECT 7536(T)).
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