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Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay

Published on: June 7, 2020

An estrogen sensor for poultry sex sorting.

H T Tran¹, W Ferrell, T R Butt

¹LifeSensors Inc., 271 Great Valley Parkway, Malvern, PA 19355, USA. butt@lifesensors.com

Journal of Animal Science

|January 19, 2010

Summary

This summary is machine-generated.

A new yeast-based assay can rapidly and affordably determine the sex of poultry eggs before hatching. This automated method addresses challenges with manual sexing, offering a scalable solution for the poultry industry.

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Published on: May 24, 2014

Area of Science:

Agricultural Science
Biotechnology
Animal Science

Background:

Poultry production requires accurate sex determination for efficient management due to sex-specific growth rates and nutritional needs.
Manual sexing of newly hatched poultry is labor-intensive, costly, and declining in workforce availability.
Current manual methods face challenges including repetitive motion disorders and scalability for millions of birds daily.

Purpose of the Study:

To develop an automated, rapid, and cost-effective method for poultry egg sex sorting before hatching.
To address the limitations and challenges associated with traditional manual sex determination in the poultry industry.
To introduce a novel technology for standardizing and automating the sex sorting process.

Main Methods:

Development of a yeast-based assay to detect sex-specific differences in embryonated eggs.
Quantification of estrogen concentration in the allantoic fluid of eggs.
Utilizing estrogen levels to differentiate between male and female embryos.

Main Results:

A facile, rapid, and low-cost assay was successfully developed.
The assay effectively distinguishes male from female embryonated eggs based on estrogen levels.
The technology demonstrates potential for accurate pre-hatch sex determination.

Conclusions:

The novel yeast-based assay offers a viable solution for automated poultry egg sex sorting.
This technology can potentially resolve issues related to manual sexing, including cost and labor.
The method promises to standardize and automate sex sorting, improving efficiency in the poultry industry.