Related Experiment Video

Updated: Jun 17, 2026

In vivo and in vitro Studies of Adaptor-clathrin Interaction
17:14

In vivo and in vitro Studies of Adaptor-clathrin Interaction

Published on: January 26, 2011

The Rad9A checkpoint protein is required for nuclear localization of the claspin adaptor protein

Megan L Sierant1, Nicole E Archer, Scott K Davey

  • 1Department of Cancer Biology and Genetics, Cancer Research Institute, Queen's University, Kingston, ON, Canada.

Cell Cycle (Georgetown, Tex.)
|January 19, 2010
PubMed

Insights

Rad9A protein localization is crucial for Claspin

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • The Chk1-mediated DNA damage checkpoint is vital for genomic stability.
  • The interaction between Rad9A and Claspin is essential for Chk1 activation.
  • The precise role of Rad9A in regulating Claspin during the checkpoint response remains unclear.

Purpose of the Study:

  • To characterize the Rad9A-Claspin interaction under various conditions.
  • To investigate the effect of Rad9A on Claspin's cellular localization.
  • To elucidate the role of Rad9A in facilitating Claspin localization to DNA damage sites.

Main Methods:

  • Experimental verification using a Rad9A-null cell line.
  • Reconstitution experiments with wild-type Rad9A.
  • Analysis of Rad9B's role in Claspin localization in meS cells.

Main Results:

  • The Rad9A-Claspin interaction is stable under diverse conditions.
  • Nuclear localization of Rad9A influences Claspin's cellular localization.
  • Rad9B, a Rad9A paralog, also affects Claspin localization in meS cells.

Conclusions:

  • Rad9A plays a significant role in directing Claspin to sites of DNA damage.
  • Rad9A-mediated Claspin localization is a critical step for initiating the Chk1 checkpoint response.
  • Understanding this interaction is key to comprehending DNA damage response pathways.

More Related Videos

Heterokaryon Technique for Analysis of Cell Type-specific Localization09:31

Heterokaryon Technique for Analysis of Cell Type-specific Localization

Published on: March 11, 2011

Visualization of DNA Repair Proteins Interaction by Immunofluorescence07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Related Concept Videos

The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Nuclear Localization Signals and Import01:46

Nuclear Localization Signals and Import

Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
Directionality of Nuclear Transport01:42

Directionality of Nuclear Transport

Ras-related nuclear protein or Ran is a small G protein that cycles between its GTP and GDP bound states. Ran specific regulators, a Ran GTPase Activating Protein or RanGAP present in the cytosol and a Ran guanine nucleotide exchange factor or RanGEF present inside the nucleus regulate GTP/GDP exchange. A high concentration of GTP inside the cells, in addition to this asymmetric distribution of  Ran-specific regulators, leads to a higher RanGTP concentration inside the nucleus. This...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...