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Updated: Jun 17, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
Parallel, tag-directed assembly of locally derived short sequence reads
Joseph B Hiatt1, Rupali P Patwardhan, Emily H Turner
1Department of Genome Sciences, University of Washington, Seattle, Washington, USA. jbhiatt@u.washington.edu
Abstract:
We demonstrate subassembly, an in vitro library construction method that extends the utility of short-read sequencing platforms to applications requiring long, accurate reads. A long DNA fragment library is converted to a population of nested sublibraries, and a tag sequence directs grouping of short reads derived from the same long fragment, enabling localized assembly of long fragment sequences. Subassembly may facilitate accurate de novo genome assembly and metagenome sequencing.
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