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Related Concept Videos

Long-term Potentiation01:25

Long-term Potentiation

Long-term potentiation, or LTP, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTP is the process of synaptic strengthening that occurs over time between pre and postsynaptic neuronal connections. The synaptic strengthening of LTP works in opposition to the synaptic weakening of long-term depression (LTD) and together are the main mechanisms that underlie learning and memory.
Hebbian LTP
LTP can occur when presynaptic neurons...

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Recording Network Activity in Spinal Nociceptive Circuits Using Microelectrode Arrays
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TRPC channels underlie cholinergic plateau potentials and persistent activity in entorhinal cortex.

Zizhen Zhang1, Antonio Reboreda, Angel Alonso

  • 1Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec H3A 2B4 Canada.

Hippocampus
|January 19, 2010
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Summary

Transient storage of memory traces in the entorhinal cortex relies on persistent neuronal activity. This study identifies Transient Receptor Potential Canonical (TRPC) channels as key mediators of this crucial working memory mechanism.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Computational Neuroscience

Background:

  • Persistent neuronal activity is crucial for working memory, enabling transient memory trace storage in the entorhinal cortex.
  • Nonsynaptic plateau potentials, induced by acetylcholine, underlie persistent firing in various brain regions.
  • The specific cation channels responsible for these intrinsic neuronal properties remain unidentified.

Purpose of the Study:

  • To identify the cation channels responsible for acetylcholine-induced persistent neuronal firing in the medial entorhinal cortex.
  • To elucidate the role of these channels in working memory mechanisms.

Main Methods:

  • Electrophysiological recordings in layer V of rat medial entorhinal cortex.
  • Pharmacological manipulation using channel blockers (FFA, 2-APB, SKF-96365, ruthenium red) and activators (OAG).
  • Investigation of signaling pathways involving phospholipase C and intracellular calcium.
  • Peptide inhibition targeting TRPC channel interactions with PDZ proteins.

Main Results:

  • Muscarinic receptor-evoked plateau potentials and persistent firing depend on phospholipase C activation and permissive intracellular calcium levels.
  • Nonselective cation channel blockers and the TRPC channel blocker SKF-96365 suppressed persistent activity.
  • A carbachol-activated, nonselective cationic current with a TRPC-like phenotype was observed.
  • Inhibition of TRPC4/5 subunit interactions with PDZ proteins disrupted plateau potentials and persistent firing.

Conclusions:

  • Transient Receptor Potential Canonical (TRPC) cation channels are critical for mediating persistent muscarinic currents in entorhinal cortex projection neurons.
  • These TRPC channels significantly contribute to the firing properties and mnemonic functions of these neurons, supporting working memory.