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Related Experiment Video

Updated: Jun 17, 2026

In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity

In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity

Published on: January 5, 2016

How do nematodes transfer phosphorylcholine to carbohydrates?

William Harnett¹, Justyna Rzepecka, Katrina M Houston

¹Strathclyde Institute of Pharmacy and Biomedical Sciences, Glasgow, UK. w.harnett@strath.ac.uk

Trends in Parasitology

|January 20, 2010

Summary

Nematodes attach phosphorylcholine (PC) to carbohydrates, crucial for development and immune evasion. This study suggests the PC transferase enzyme may belong to the fukutin family, found across species.

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Area of Science:

Biochemistry
Molecular Biology
Parasitology

Background:

Nematodes exhibit unique phosphorylcholine (PC) conjugation to carbohydrates.
This conjugation is vital for nematode development and immune modulation in parasitic species.

Purpose of the Study:

To investigate the biosynthetic pathway of nematode PC-containing glycoconjugates.
To identify the specific enzyme responsible for transferring PC to carbohydrates (PC transferase).

Main Methods:

Review of existing literature on nematode glycoconjugate biosynthesis.
Comparative analysis of enzyme families with phosphoryl-ligand transferase activity.

Main Results:

The study focuses on the final enzymatic step in PC-glycoconjugate formation.
Hypothesizes that the PC transferase is a member of the fukutin family of enzymes.

Conclusions:

The fukutin family, known for phosphoryl-ligand transferase activity, is a strong candidate for the nematode PC transferase.
This enzyme family is conserved across diverse organisms from bacteria to humans.