Related Experiment Video
Updated: Jun 16, 2026
Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
Published on: January 3, 2019
A new evaluation technique for the detection of impurities in purified proteins via CE with native UV-LIF
Audrey Rodat1, Bettina Couderc, Bernard Feurer
1Laboratoire des IMRCP, Université de Toulouse, Université Paul Sabatier, Toulouse, France.
Abstract:
An analytical methodology for quality control analyses of IgG and their impurities is presented using a new UV-LIF (266 nm) detector inside the cassette of a CE instrument and its performance was evaluated. The observed sensitivity was very close to that obtained by silver staining of slab gels (LOD of 25 ng/mL), while the sensitivity of the analysis is 80 times better than with CE/UV absorption (214 nm). Examples of the analysis of pharmaceutical and other commercial IgGs are provided and the kinetics of the reduction of IgG by beta-mercaptoethanol is reported, demonstrating the ease of performing the analysis.
