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Updated: Jun 16, 2026
In Vitro ELISA Test to Evaluate Rabies Vaccine Potency
Published on: May 11, 2020
[Comparison and evaluation of enzyme-linked immunization assay kits with plague reduction neutralization test for
Nai-ying Mao1, Zhen Zhu, Xiao-hong Jiang
1World Health Organization Western Pacific Region Regional Reference Measles Laboratory State Key Laboratory for Molecular Virology & Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Objective:
To evaluate and comparison of 2 commercial ELISA kits (Vrion/Serion kit and IBL kit) which ELISA kit will be used in sero-epidemiological survey in China in 2006 by Plaque Reduction Neutralization Test (PRNT).
Methods:
52 serum panel which contain different levels of measles antibody were used.
Results:
The results showed that the sensitivity, PPV and accurate of Virion/Serion kit achieved highest score when taking PRNT as "gold standard". Virion/Serion kit showed good relationship with PRNT titer: the correlation coefficients are 0.878 (P<0.01). IBL kit get lower correlation coefficients 0.850 (P<0.01). The test value and mean of unit of serum antibody increased along with neutralizing titers increased.
Conclusion:
The Virion/Serion kit is good in sensitivity and specificity. It has quantitative for single well. The kit is suitable for sera epidemiology survey to detection of automatic workstation in China.
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