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Updated: Jun 16, 2026
04:11
Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
Published on: December 15, 2023
[Establishment of hepatitis B virus marker panels]
Yong Zhang1, Feng Wang, Jing-Chen Ma
1State Key Laboratory for Infectious Disease Control and Prevention, Chinese Center for Disease Control and Prevention, National Institute for Viral Disease Control and Prevention, Beijing 100052, China.
Zhongguo Yi Miao He Mian Yi
|January 21, 2010
Summary
Hepatitis B virus (HBV) marker panels were established using repeated testing of plasma specimens. These panels aid in accurate HBV diagnostics but should be used cautiously due to potential limitations.
Area of Science:
- Hepatology
- Immunology
- Clinical Diagnostics
Context:
- Accurate detection of Hepatitis B virus (HBV) markers is crucial for diagnosis and management.
- Standardized panels are essential for quality control and validation of diagnostic assays.
Purpose:
- To establish reliable Hepatitis B virus (HBV) marker panels for diagnostic quality assurance.
- To evaluate the feasibility of creating panels for multiple HBV serological markers.
Summary:
- 155 plasma specimens were tested twice for 5 HBV markers using Abbott MEIA kits.
- Panels for HBsAg, anti-HBs, anti-HBc, and anti-HBe were successfully established with high concordance.
- A panel for HBeAg could not be established due to insufficient positive results.
Impact:
- The study demonstrates a method for creating HBV marker panels through rigorous screening.
- Established panels can enhance the reliability of HBV diagnostic testing.
- Careful application of these panels is recommended to minimize diagnostic errors.
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