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Updated: Jun 16, 2026

Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses
Published on: May 2, 2019
[Gene identification of mumps wild virus isolated firstly in Jilin province]
Shuang Wang1, Xin Chang, Jian-Hui Zhou
1Jilin Provincial Center for Disease Control and Prevention,Key Laboratory of Health Bureau ofJilin Province, Changchun 130062, Jilin, China.
Objective:
To observe Cytopathic Effect of wild-type mump viruses in Vero/SLAM cell and identify genotype in a mump outbreak in 2008.
Methods:
Throat swabs were collected and to isolated mump virus with Vero/slam cells.Cytopathic effect was observed and determined mump virus type with Reverse Transcription-Polymerase Chain Reaction (RT-PCR).
Results:
Of the total 15 throat swabs, different CPE were observed in 3 throat swabs but genotypes are same, they are F type, 3 throat swabs were not observed cytopathic effect but genotypes were F type.
Conclusion:
There were different kinds of cytopathic effect in one mump outbreak although genotypes were same.

