Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 16, 2026

Imaging Protein-protein Interactions in vivo
11:15

Imaging Protein-protein Interactions in vivo

Published on: October 10, 2010

Imaging macromolecular interactions at an interface.

Joshua W Lampe1, Zhengzheng Liao, Ivan J Dmochowski

  • 1Center for Resuscitation Science, Department of Emergency Medicine, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.

Langmuir : the ACS Journal of Surfaces and Colloids
|January 21, 2010
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proceedings from the 2025 Midwest Pediatric Device Consortium showcase featuring software as a medical device.

BMC proceedings·2026
Same author

Gas Embolism: Fundamentals, Diagnosis, and Treatment.

IEEE reviews in biomedical engineering·2026
Same author

Cavity acidification limits ferritin iron biomineralization.

Journal of inorganic biochemistry·2026
Same author

Construction of multi-component covalent organic framework coated stainless steel fiber for efficient microextraction of psychiatric drugs: experiments and clinical applications.

Analytica chimica acta·2026
Same author

<i>Lygodium japonicum</i> Herb Ameliorates Nonalcoholic Fatty Liver Disease by Inhibiting Hepatic Lipid Accumulation and Inflammatory Response in ob/ob Mice.

ACS omega·2026
Same author

Caffeine, MitoQ, and GABA Prophylaxis of Mitochondrial Dysfunction Induced in Human Pulmonary Cells by Normobaric-Hyperoxia and Hyperbaric-Hyperoxia.

Oxidative medicine and cellular longevity·2025
Same journal

Laser-Assisted Electrochemical Deposition of Bilateral Au Coatings on Ni Foils: Mechanism and Experimental Study.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Mechanistic Insights into Pulmonary Surfactant Inactivation.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

MPN-GE Bilayer Interphase Construction: Green Modification Derived from Biomass and Synergistic Enhancement of CFRP.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Magnetically Retrievable Core@Shell Nanocomposites for Rare Earth Element Adsorption: Experimental and Machine Learning Insights.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Microstreaming of a Pneumatically Controlled Bubble under Hydrostatic Pressure and Crossflow.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Tuning Pore Sizes of Core-Shell Dendritic Mesoporous Silica Nanoparticles for Efficient Loading of Functional Materials.

Langmuir : the ACS journal of surfaces and colloids·2026
See all related articles

Confocal laser scanning microscopy quantifies protein behavior at interfaces. Albumin and fibrinogen show distinct interactions, with surfactants inhibiting protein adsorption to the gas-liquid interface.

Area of Science:

  • Biophysics
  • Surface Chemistry
  • Biotechnology

Background:

  • Physiological, pathological, and technological processes depend on interfaces.
  • Quantifying molecular events at interfaces is crucial but challenging.

Purpose of the Study:

  • To develop a model-independent method for measuring protein concentration and mobility at interfaces.
  • To investigate the behavior of albumin and fibrinogen at the gas-liquid interface.

Main Methods:

  • Utilized confocal laser scanning microscopy (CLSM) with fluorescently labeled proteins.
  • Studied albumin and fibrinogen individually, together, and with a surfactant (Pluronic F-127) in aqueous droplets.
  • Measured molecular behaviors, surface inhomogeneity, and partitioning between bulk and interface.

More Related Videos

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces
08:05

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces

Published on: September 9, 2022

Related Experiment Videos

Last Updated: Jun 16, 2026

Imaging Protein-protein Interactions in vivo
11:15

Imaging Protein-protein Interactions in vivo

Published on: October 10, 2010

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces
08:05

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces

Published on: September 9, 2022

Main Results:

  • Demonstrated distinct behaviors of albumin and fibrinogen at the gas-liquid interface.
  • Showed that adsorption from multi-species solutions differs significantly from single-species adsorption.
  • Confirmed that surfactants can inhibit protein occupation of the interface.

Conclusions:

  • CLSM provides a robust method for analyzing interfacial protein dynamics.
  • Protein adsorption at interfaces is complex and influenced by species composition and presence of surfactants.
  • Understanding these interfacial phenomena is key for various scientific and technological applications.