Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 16, 2026

Viability Assays for Cells in Culture
12:03

Viability Assays for Cells in Culture

Published on: January 20, 2014

Adherent cell assay results affected by variable z-position mixing.

Nelson Carramanzana1, Sandra Ross, Gloria Biddlecombe

  • 1Research and Automation Technologies, Amgen, Thousand Oaks, California 91320, USA. nelsonc@amgen.com

Assay and Drug Development Technologies
|January 21, 2010
PubMed
Summary

Modifying mixing dynamics significantly impacts cell assays. Variable z-position (VZP) mixing prevents artifacts, improving imaging and protein expression quantification in cellular assays.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Anti-GAD antibody-associated epilepsy in pregnancy.

Obstetric medicine·2026
Same author

Source apportionment of fine particulate matter (PM<sub>2.5</sub>) personal exposures: findings from the Household Air Pollution Intervention Network (HAPIN) study in rural Guatemala.

Journal of exposure science & environmental epidemiology·2026
Same author

Comparing Performance and Reliability of Collocated Enhanced Children's MicroPEM (ECM) on Gravimetric and Nephelometric PM<sub>2.5</sub> Personal Exposure Samples in Field Measurements in Rural Guatemala.

Indoor air·2026
Same author

Characterizing household air pollutant concentrations associated with an electrification program in the rural San Joaquin Valley.

Environmental research, health : ERH·2026
Same author

Precision Glyco-Modulated GcMAF2.0 Targets CLEC10A to Bidirectionally Re-Wire Macrophage States: Cross-Species Evidence, Mechanistic Omics and Translational Horizons.

Immunology·2026
Same author

Adjunct EF-M2 therapy improves clinical activity, steroid-sparing, and macrophage-linked biomarkers in feline chronic enteropathy: A randomized, double-blind, and placebo-controlled trial.

Veterinary world·2026

Area of Science:

  • Cell Biology
  • Biotechnology
  • Assay Development

Background:

  • Mixing dynamics during reagent addition can introduce artifacts in cell-based assays.
  • Standard mixing protocols may affect cell morphology, protein expression, and assay results.

Purpose of the Study:

  • To investigate the impact of mixing dynamics on cell morphology and protein expression.
  • To evaluate Variable z-position (VZP) mixing as a method to mitigate assay artifacts.

Main Methods:

  • Tested four adherent cell types using VZP mixing.
  • Assessed effects of VZP on quantitative imaging, protein expression, cell viability, and morphology.
  • Utilized phalloidin-actin staining and green fluorescence protein (GFP) fusion proteins for analysis.

More Related Videos

Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System
04:47

Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System

Published on: May 22, 2020

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface
13:22

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface

Published on: November 2, 2011

Related Experiment Videos

Last Updated: Jun 16, 2026

Viability Assays for Cells in Culture
12:03

Viability Assays for Cells in Culture

Published on: January 20, 2014

Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System
04:47

Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System

Published on: May 22, 2020

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface
13:22

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface

Published on: November 2, 2011

Main Results:

  • VZP mixing improved cytoplasmic fluorescence quantitation by 47% in phalloidin-actin assays.
  • Reduced fluorescence variability in GFP-expressing cells by 15%.
  • No significant impact on cell viability, EC50 values, or assay curve patterns was observed.

Conclusions:

  • Variable z-position (VZP) mixing effectively eliminates artifactual effects caused by organic solute addition.
  • VZP is a valuable systematic process for cellular assay workflows, enhancing data accuracy and reproducibility.
  • This method improves the reliability of quantitative imaging and protein expression measurements in cell-based assays.