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Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

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Related Experiment Video

Updated: Jun 16, 2026

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
07:16

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

A new DNA combing method for biochemical analysis.

Masahiko Oshige1, Kouji Yamaguchi, Shun-Ichi Matsuura

  • 1Department of Chemical and Environmental Engineering, Graduate School of Engineering, Gunma University, Gunma 376-8515, Japan.

Analytical Biochemistry
|January 21, 2010
PubMed
Summary

A new moving droplet method stretches DNA molecules for biochemical analysis using minimal sample volumes. This technique enables single-molecule analysis of DNA synthesis by DNA polymerases.

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Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
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Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Published on: May 6, 2010

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Last Updated: Jun 16, 2026

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
07:16

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
22:27

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Published on: May 6, 2010

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biophysics

Background:

  • Molecular combing techniques are crucial for DNA analysis.
  • Existing methods often require large sample volumes, limiting their application.
  • There is a need for simpler, more efficient DNA stretching methods.

Purpose of the Study:

  • To develop a novel, simple molecular combing method for biochemical reaction analysis.
  • To demonstrate the utility of this method for single-molecule DNA analysis.

Main Methods:

  • Developed the 'moving droplet method' utilizing a sloped glass substrate.
  • Small droplets containing DNA molecules create a moving interface to stretch DNA.
  • Applied the method to stretch and immobilize lambda DNA molecules.

Main Results:

  • The moving droplet method effectively stretches DNA molecules.
  • The method requires significantly smaller sample volumes compared to traditional techniques.
  • Successfully performed single-molecule analysis of DNA synthesis by DNA polymerases.

Conclusions:

  • The moving droplet method is a simple and efficient technique for DNA stretching.
  • Its low sample volume requirement broadens its applicability in various scientific fields.
  • This method facilitates advanced single-molecule analysis of enzymatic reactions like DNA synthesis.