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Related Experiment Video

Updated: Jun 16, 2026

Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments
07:24

Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments

Published on: December 31, 2016

Working with OpusXpress: methods for high volume oocyte experiments.

Roger L Papke1, Clare Stokes

  • 1Department of Pharmacology and Therapeutics, University of Florida, College of Medicine, Gainesville, FL 32610, USA. rlpapke@ufl.edu

Methods (San Diego, Calif.)
|January 21, 2010
PubMed
Summary

This study details the use of the OpusXpress system for high-throughput Xenopus oocyte voltage clamp recordings. It offers practical insights and standard operating procedures for ion channel research and drug discovery.

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Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments
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Area of Science:

  • Pharmacology
  • Molecular Biology
  • Biophysics

Background:

  • High-throughput electrophysiology is crucial for ion channel research and drug discovery.
  • Xenopus oocytes are a widely used system for heterologous gene expression and functional studies.
  • Semi-automated systems can improve efficiency in voltage clamp recordings.

Purpose of the Study:

  • To share practical experience and standard operating procedures for the OpusXpress recording system.
  • To provide guidance on large-volume Xenopus oocyte handling for heterologous expression.
  • To offer insights applicable to any group utilizing Xenopus oocytes for ion channel research.

Main Methods:

  • Field testing of an OpusXpress prototype and subsequent use of an early production model.
  • Conducting thousands of voltage clamp experiments over seven years.
  • Collaborative research in drug discovery utilizing the system.

Main Results:

  • Extensive publication record stemming from OpusXpress system usage.
  • Successful implementation of large-volume oocyte handling protocols.
  • Demonstrated utility of the system in drug discovery research.

Conclusions:

  • The OpusXpress system is a valuable tool for high-throughput Xenopus oocyte voltage clamp recordings.
  • The shared procedures and team organization can benefit researchers using Xenopus oocytes.
  • The system facilitates efficient ion channel research and drug discovery.