Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Catenins01:23

Catenins

Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the adherens...
Structure of Cadherins01:25

Structure of Cadherins

The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins”   is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This diversity of cadherins...
Cadherins in Tissue Organization01:19

Cadherins in Tissue Organization

The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
Tension Response at Adherens Junctions01:26

Tension Response at Adherens Junctions

The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin homology) domains...
Adherens Junctions01:24

Adherens Junctions

Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types –  adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as  epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as  heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
The endothelial cells...
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Constructing an evaluation system for the whole process of urban domestic waste disposal technology in high-altitude areas based on multiple analysis methods: Taking Lhasa as an example.

Waste management & research : the journal of the International Solid Wastes and Public Cleansing Association, ISWA·2026
Same author

Single-dose dual-indication nanoparticle vaccine co-displaying <i>Pseudomonas aeruginosa</i> antigen and tetanus toxin fragment confers durable protection against mixed exposure.

Materials today. Bio·2026
Same author

An Integrative Strategy Delineates Modular Metabolic Remodeling and Potential Therapeutic Targets Across Metabolic Diseases.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Bioaccessibility and Dynamic Changes in Free and Bound Phenolics in Rice Bean (<i>Vigna umbellata</i>) During Simulated Digestion.

Foods (Basel, Switzerland)·2026
Same author

Multi-scale photon-efficient depth reconstruction based on spatiotemporal priors.

Optics express·2026
Same author

Machine learning-driven conditional survival prediction model for atypical teratoid/rhabdoid tumor.

Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery·2026

Related Experiment Video

Updated: Jun 16, 2026

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
08:15

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Published on: October 17, 2014

Vinculin regulates cell-surface E-cadherin expression by binding to beta-catenin.

Xiao Peng1, Laura E Cuff, Cort D Lawton

  • 1Department of Biochemistry, University of Iowa Roy J. Carver College of Medicine, Iowa City, IA 52242, USA.

Journal of Cell Science
|January 21, 2010
PubMed
Summary

Vinculin is crucial for cell-cell adhesion by stabilizing E-cadherin at the cell surface. This interaction, mediated by beta-catenin, is essential for maintaining epithelial cell junctions and normal cell morphology.

More Related Videos

Reconstitution Of &#946;-catenin Degradation In Xenopus Egg Extract
09:41

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract

Published on: June 17, 2014

Related Experiment Videos

Last Updated: Jun 16, 2026

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
08:15

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Published on: October 17, 2014

Reconstitution Of &#946;-catenin Degradation In Xenopus Egg Extract
09:41

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract

Published on: June 17, 2014

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Vinculin's role in adherens junctions has been unclear despite its known presence.
  • Previous studies could not isolate vinculin's function at cell-cell junctions from cell-matrix adhesions.

Purpose of the Study:

  • To elucidate the specific function of vinculin in cell-cell junctions.
  • To investigate the mechanism by which vinculin regulates E-cadherin at the cell surface.

Main Methods:

  • Developed a short hairpin RNA (shRNA)-based knockdown/substitution system to specifically target vinculin at cell-cell adhesions.
  • Utilized epithelial cells to assess the impact of vinculin perturbation on cell morphology and adhesion.
  • Employed vinculin and beta-catenin mutants to analyze binding interactions and rescue experiments.

Main Results:

  • Perturbing vinculin at cell-cell junctions in epithelial cells altered cell morphology and reduced cadherin-dependent adhesion.
  • These defects were linked to decreased E-cadherin expression at the cell surface.
  • Restoring vinculin, but not a beta-catenin binding-defective mutant, rescued E-cadherin surface expression.

Conclusions:

  • Vinculin is a novel regulator of E-cadherin function at cell-cell junctions.
  • The interaction between vinculin and beta-catenin is critical for stabilizing E-cadherin at the cell surface.
  • This study provides new insights into the dynamic regulation of adherens junctions.