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Related Experiment Video

Updated: Jun 16, 2026

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
05:46

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Published on: June 9, 2020

Assessing the application of tissue microarray technology to kidney research.

Ming-Zhi Zhang1, Yinghao Su, Bing Yao

  • 1Vanderbilt O'Brien Center, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee, USA. ming-zhi.zhang@vanderbilt.edu

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|January 21, 2010
PubMed
Summary

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Tissue microarray (TMA) technology is validated for rodent kidney studies. This high-throughput method enables simultaneous protein expression analysis in mouse and rat kidneys under various conditions.

Area of Science:

  • Nephrology
  • Biotechnology
  • Molecular Biology

Background:

  • Tissue microarray (TMA) is a high-throughput method for analyzing protein expression across multiple samples.
  • TMA technology has not been previously applied to physiological and pathophysiological studies of rodent kidneys.

Purpose of the Study:

  • To evaluate the validity and reliability of using TMA for assessing protein expression in mouse and rat kidneys.
  • To establish a kidney TMA technique for diverse physiological and pathophysiological conditions.

Main Methods:

  • Developed a representative TMA block including mouse and rat kidney tissues (cortex, outer medulla, inner medulla) with varied fixatives and developmental stages.
  • Included kidneys from animals under different physiological or pathophysiological treatments, along with built-in controls.

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A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
09:32

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

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Last Updated: Jun 16, 2026

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
05:46

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Published on: June 9, 2020

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
09:32

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

  • Performed immunostaining for key kidney proteins (e.g., cyclooxygenase-2, renin, aquaporin-2) on kidney TMA slides.

    • Main Results:

    • Quantitative analysis of cyclooxygenase-2 expression demonstrated that individual TMA cores accurately represent protein expression compared to whole-kidney sections.
    • Successful immunostaining for multiple proteins confirmed the utility of the kidney TMA approach.
    • The developed TMA block effectively accommodates diverse tissue types and experimental conditions.

    Conclusions:

    • Kidney TMA is a valid and reliable technique for assessing protein expression in rodent kidneys.
    • This method is a promising tool for investigating protein expression profiles under various physiological and pathophysiological conditions.
    • The established kidney TMA technique facilitates high-throughput analysis in nephrology research.