Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Unraveling the joints: a narrative review of osteoarthritis.

European review for medical and pharmacological sciences·2024

Same author

Solving classification tasks by a receptron based on nonlinear optical speckle fields.

Neural networks : the official journal of the International Neural Network Society·2023

Same author

FOCUS: fast Monte Carlo approach to coherence of undulator sources.

Journal of synchrotron radiation·2023

Same author

Correction to: Robust Institutional Support and Collaboration Between Summer Training Programs in Cancer and Biomedicine Drive the Pivot to a Virtual Format in Response to the COVID Pandemic.

Journal of cancer education : the official journal of the American Association for Cancer Education·2022

Same author

Robust Institutional Support and Collaboration Between Summer Training Programs in Cancer and Biomedicine Drive the Pivot to a Virtual Format in Response to the COVID Pandemic.

Journal of cancer education : the official journal of the American Association for Cancer Education·2022

Same author

The pursuit of stability in halide perovskites: the monovalent cation and the key for surface and bulk self-healing.

Materials horizons·2021

Search research articles

Related Experiment Video

Updated: Jun 16, 2026

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

Confocal zero-angle dynamic depolarized light scattering.

M A C Potenza¹, T Sanvito, M D Alaimo

¹Dipartimento di Fisica, Università di Milano, Italy. marco.potenza@unimi.it

The European Physical Journal. E, Soft Matter

|January 21, 2010

Summary

This summary is machine-generated.

We developed a new Dynamic Depolarized Scattering technique that accurately measures particle diffusion and birefringence, even in highly turbid samples. This method overcomes limitations of previous techniques, enabling broader applications in material science.

More Related Videos

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Published on: August 11, 2020

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Published on: September 8, 2016

Related Experiment Videos

Last Updated: Jun 16, 2026

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Published on: August 11, 2020

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Published on: September 8, 2016

Area of Science:

Physics
Materials Science
Physical Chemistry

Background:

Dynamic light scattering is a powerful technique for characterizing particle dynamics.
Traditional methods struggle with highly turbid samples due to multiple scattering.
Measuring intrinsic birefringence requires specialized techniques.

Purpose of the Study:

To introduce a novel Dynamic Depolarized Scattering (DDS) method.
To enable accurate measurements in highly turbid, non-index-matched samples.
To simultaneously determine rotational and translational diffusion coefficients and intrinsic birefringence.

Main Methods:

Implementation of a tight confocal, zero scattering angle, heterodyne detection scheme.
Utilizing number fluctuation spectroscopy for translational diffusion measurements.
Analyzing the amplitude ratio of rotational and translational modes for birefringence determination.

Main Results:

The DDS method demonstrates high immunity to parasitic multiple-scattering contributions.
Successful operation with non-index-matched samples exhibiting large turbidity.
Accurate determination of both rotational and translational diffusion coefficients.
Quantification of intrinsic particle birefringence from mode amplitude ratios.

Conclusions:

The novel DDS technique offers a robust solution for studying particle dynamics in complex, turbid media.
This method expands the applicability of light scattering techniques to a wider range of materials.
Simultaneous measurement of diffusion and birefringence provides comprehensive particle characterization.