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Updated: Jun 16, 2026

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Published on: July 22, 2013

Yeast based sensors.

Mifumi Shimomura-Shimizu¹, Isao Karube

¹School of Bioscience and Biotechnology, Tokyo University of Technology, Hachioji, Japan.

Advances in Biochemical Engineering/Biotechnology

|January 21, 2010

Summary

This summary is machine-generated.

Yeast based sensors, utilizing immobilized yeast and DO electrodes, offer versatile analytical tools. These sensors are effective for environmental monitoring and biochemical process control.

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In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

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Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

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Area of Science:

Biotechnology
Environmental Science
Analytical Chemistry

Background:

Microbial cell sensors, pioneered in 1977, have evolved significantly.
Yeast, facultative anaerobes, exhibit adaptability to various analytes.
Yeast based sensors combine DO electrodes with immobilized yeast for analysis.

Purpose of the Study:

To review the principles and applications of yeast based sensors.
To highlight the adaptability of different yeast species in sensor development.
To showcase the utility of yeast sensors in environmental and industrial settings.

Main Methods:

Immobilization of omnivorous yeast onto a DO electrode.
Utilizing yeast's facultative anaerobic properties for detection.
Employing specific yeast strains like Trichosporon cutaneum for targeted analysis.

Main Results:

Demonstrated suitability of yeast based sensors for online process control.
Successful application of Trichosporon cutaneum for industrial wastewater organic pollution estimation.
Versatility of yeast sensors across diverse analytical applications.

Conclusions:

Yeast based sensors are valuable analytical tools with broad applicability.
Their adaptability makes them suitable for environmental monitoring and biochemical process control.
Further development continues to enhance their analytical capabilities.