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Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
Published on: August 6, 2011
Multiplex real-time PCR using SYBR® GreenER™ for the detection of DNA allergens in food
Simona Pafundo1, Mariolina Gullì, Nelson Marmiroli
1Department of Environmental Sciences, Division of Genetics and Environmental Biotechnology, University of Parma, Viale G.P. Usberti 11/A, 43100, Parma, Italy.
Abstract:
We describe the development of a six-target real-time multiplex PCR assay with the SYBR® GreenER™ fluorescent dye, targeted to genes encoding for allergenic proteins commonly present in many processed food products (patent application pending). The assay was successfully trialled on reconstructed food matrices and on a range of commercial foodstuffs, and is proposed as a ready-to-use analytical tool for food manufacturers to detect the presence or confirm the absence of sequences encoding for important allergenic proteins of plant origin.
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