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Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

EPS and iPS Cells in Disease Research

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...

Somatic to iPS Cell Reprogramming

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

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Related Experiment Video

Updated: Jun 16, 2026

Generation of Mice Derived from Induced Pluripotent Stem Cells

Generation of Mice Derived from Induced Pluripotent Stem Cells

Published on: November 29, 2012

The mighty mice prove pluripotency for iPSCs.

¹Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China. pei_duanqing@gibh.ac.cn

Journal of Molecular Cell Biology

|January 22, 2010

Summary

Induced pluripotent stem cells (iPSCs) injected into tetraploid blastocysts successfully generated live pups, proving their pluripotency. This breakthrough validates iPSC technology for regenerative medicine applications.

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Last Updated: Jun 16, 2026

Generation of Mice Derived from Induced Pluripotent Stem Cells

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Published on: November 29, 2012

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

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Published on: February 28, 2017

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

Published on: September 6, 2014

Area of Science:

Stem cell biology
Developmental biology
Regenerative medicine

Background:

Induced pluripotent stem cells (iPSCs) hold promise for regenerative medicine.
Assessing the full pluripotency of iPSCs in vivo is crucial for their clinical application.

Purpose of the Study:

To demonstrate that iPSCs are as pluripotent as embryonic stem cells.
To validate iPSC technology for potential use in regenerative medicine.

Main Methods:

Injection of iPSCs into tetraploid blastocysts.
Generation of live pups from tetraploid embryos reconstructed with iPSCs.

Main Results:

Successfully generated live newborn mice from injected iPSCs.
Demonstrated that iPSCs possess developmental potential equivalent to embryonic stem cells.

Conclusions:

iPSCs are fully pluripotent and can generate all cell types in a live organism.
This study validates the potential of iPSC technology for regenerative therapies.