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Polymorphism in merozoite surface protein-1 gene in north & northwest Indian field isolates of Plasmodium vivax

Umar Farooq1, N Malla, M L Dubey

  • 1Department of Parasitology, Postgraduate Institute of Medical Education & Research, Chandigarh 160 012, India.

The Indian Journal of Medical Research
|January 22, 2010
PubMed
Abstract

Insights

Extensive genetic diversity was found in the Pvmsp-1 gene of Plasmodium vivax isolates from India. This significant polymorphism in the merozoite surface protein-1 gene impacts malaria vaccine development strategies.

Area of Science:

  • Molecular Biology
  • Parasitology
  • Immunology

Background:

  • Merozoite surface protein-1 of Plasmodium vivax (Pvmsp-1) is a key vaccine candidate.
  • Polymorphism in the msp-1 gene is significant for vaccine efficacy.
  • Limited data exists on P. vivax msp-1 gene polymorphism in Indian isolates.

Purpose of the Study:

  • To investigate Pvmsp-1 gene polymorphism in Indian P. vivax isolates.
  • To assess the genetic variability of a crucial malaria vaccine target.
  • To analyze 25 P. vivax isolates from North and Northwest India.

Main Methods:

  • DNA extraction from whole blood samples.
  • Polymerase Chain Reaction (PCR) amplification of the polymorphic region-5 of Pvmsp-1.
  • Restriction Fragment Length Polymorphism (RFLP) analysis using Mva-1 enzyme and agarose gel electrophoresis.

Main Results:

  • PCR revealed 4 distinct allelic types among the 25 isolates.
  • RFLP analysis further differentiated these into 9 sub-allelic types.
  • PCR-RFLP demonstrated effectiveness in studying Pvmsp-1 gene polymorphism.

Conclusions:

  • Significant genetic polymorphism exists in the Pvmsp-1 gene of Indian P. vivax isolates.
  • Further studies with larger, geographically diverse Indian isolates are warranted.
  • Findings have implications for P. vivax malaria vaccine development strategies.

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