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Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
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Red-white-blue emission switching molecular beacons: ratiometric multicolour DNA hybridization probes
Reji Varghese1, Hans-Achim Wagenknecht
1Univeristy of Regensburg, Institute for Organic Chemistry, Universitätsstr. 31, D-93053 Regensburg, Germany.
Organic & Biomolecular Chemistry
|January 22, 2010
Abstract:
Molecular beacons with two fluorophores derived from ethynyl pyrene and ethynyl nile red exhibit red emission in the hairpin that changes to blue through white upon binding to the target sequence with a dramatic shift of approximately 225 nm.
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