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Related Experiment Video

Updated: Jun 16, 2026

Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology
12:36

Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology

Published on: March 14, 2020

Improved reproducibility in preparing precision-cut liver tissue slices.

Martina Zimmermann1, Johanna Lampe, Sebastian Lange

  • 1Department of Gastroenterology and Hepatology, University Hospital Tuebingen, Otfried-Mueller-Str. 10, 72076, Tuebingen, Germany.

Cytotechnology
|January 22, 2010
PubMed
Summary

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New vibrating blade microtomes improve precision-cut liver tissue slice (PCLS) reproducibility. This advancement enhances accuracy for PCLS used in drug metabolism, toxicology, and efficacy studies across multiple species.

Area of Science:

  • * Pharmacology and Toxicology
  • * Biomedical Engineering
  • * Cell Biology

Background:

  • * Precision-cut liver tissue slices (PCLS) are vital for studying drug metabolism, toxicology, and efficacy.
  • * Traditional slicing methods show variability, impacting research reproducibility.
  • * Standard apparatus like the Krumdieck tissue slicer has been widely used.

Purpose of the Study:

  • * To compare the reproducibility of PCLS generated by a new Leica VT1200 S vibrating blade microtome versus the standard Krumdieck tissue slicer.
  • * To assess slice thickness accuracy and tissue quality (ATP content) across different species.

Main Methods:

  • * Liver tissues from human, pig, cattle, rat, and mouse were sectioned using both Leica VT1200 S and Krumdieck slicers.

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Optimized and Simplified Technique for the Production and Culture of Precision-Cut Liver Slices
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Optimized and Simplified Technique for the Production and Culture of Precision-Cut Liver Slices

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  • * Reproducibility of slice thickness was evaluated through cross-sectioning.
  • * Adenosine triphosphate (ATP) content was measured to determine tissue viability and quality.

    • Main Results:

    • * The Leica VT1200 S demonstrated improved accuracy and reproducibility for rat, mouse, and human PCLS.
    • * Variability in slice thickness was reduced with the new vibrating blade microtome.
    • * Tissue quality, assessed by ATP content, was maintained.

    Conclusions:

    • * The Leica VT1200 S vibrating blade microtome offers superior reproducibility for generating PCLS, particularly for human, rat, and mouse tissues.
    • * This improved precision can enhance the reliability of pharmacological and toxicological studies.
    • * The new instrument represents a significant advancement over standard methods for PCLS preparation.