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Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
Published on: November 26, 2013
Shotgun mass spectrometry workflow combining IEF and LC-MALDI-TOF/TOF
Giuseppina Maccarrone1, Christoph W Turck, Daniel Martins-de-Souza
1Max Planck Institute of Psychiatry, Proteomics and Biomarkers, Kraepelinstrasse 2, 80804, Munich, Germany.
Abstract:
We present a high throughput shotgun mass spectrometry workflow using a bidimensional peptide fractionation procedure consisting of isoelectric focusing and RP-HPLC prior to mass spectrometric analysis, with the aim of optimizing peptide separation and protein identification. As part of the workflow we used the 'Isotope-Coded Protein Labeling' (ICPL) method for accurate relative quantitation of protein expression. Such workflow was successfully applied to a comparative proteome analysis of schizophrenia versus healthy control brain tissues and can be an alternative to proteome researches.
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