Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Delivery systems for gene therapy.

G Y Wu1, C H Wu

  • 1Department of Medicine, University of Connecticut School of Medicine, Farmington.

Biotherapy (Dordrecht, Netherlands)
|January 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The effect of soy isoflavone on bone mineral density in postmenopausal Taiwanese women with bone loss: a 2-year randomized double-blind placebo-controlled study.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2011
Same author

Fabrication and characterization of high-T(c) YBa(2)Cu(3)O(7-x) nanoSQUIDs made by focused ion beam milling.

Nanotechnology·2011
Same author

Thigh muscle volume predicted by anthropometric measurements and correlated with physical function in the older adults.

The journal of nutrition, health & aging·2011
Same author

Effects of operational parameters on decolorisation of C.I. Reactive Black 5 in UV/TiO(2) system.

Water science and technology : a journal of the International Association on Water Pollution Research·2011
Same author

cDNA cloning by inverse polymerase chain reaction.

Methods in molecular biology (Clifton, N.J.)·2011
Same author

Progress toward a synthetic virus : a multicomponent system for liver-directed DNA delivery.

Methods in molecular medicine·2011

Researchers developed a targeted gene delivery system using asialoglycoprotein and polycations for efficient in vivo gene expression in hepatocytes. This method enables specific liver gene delivery and expression, which can be sustained with partial hepatectomy.

Area of Science:

  • Molecular Biology
  • Gene Therapy
  • Hepatology

Background:

  • In vitro gene transfection into mammalian cells is established, but in vivo methods are challenging and lack specificity.
  • Existing in vivo gene delivery techniques are technically difficult and do not target specific cell types effectively.

Purpose of the Study:

  • To develop a soluble, targetable DNA carrier system for specific in vivo gene delivery to hepatocytes.
  • To demonstrate the feasibility of using an asialoglycoprotein-polycation conjugate for targeted gene expression in the liver.

Main Methods:

  • Constructed a DNA carrier system by covalently coupling asialoglycoprotein to a polycation.
  • Utilized the asialoglycoprotein receptor on hepatocytes for targeted cellular uptake.
  • Assessed gene delivery and expression using chloramphenicol acetyltransferase (CAT) as a marker gene in vitro and in vivo.

Related Experiment Videos

Main Results:

  • Specific in vitro CAT gene delivery and expression were observed in cell lines expressing the asialoglycoprotein receptor.
  • Intravenous injection of conjugate-DNA complexes in rats led to rapid detection of CAT DNA in the liver.
  • Significant CAT enzyme activity was detected specifically in the liver 24 hours post-injection, with transient expression observed.
  • Partial hepatectomy following DNA complex injection resulted in persistent high-level hepatic CAT activity for up to 11 weeks.

Conclusions:

  • A targetable gene delivery system using asialoglycoprotein-polycation conjugates enables efficient in vivo gene expression in the liver via simple intravenous injection.
  • Hepatocyte replication, induced by partial hepatectomy, significantly enhances and prolongs foreign gene expression in the liver.